AmScope B120C-DK-E1 Binocular LED Darkfield Microscope User Manual

導入

This manual provides instructions for the proper setup, operation, and maintenance of your AmScope B120C-DK-E1 Binocular LED Darkfield Microscope. This instrument is designed for educational, clinical, and laboratory applications, offering both brightfield and darkfield observation capabilities. Please read this manual thoroughly before using the microscope to ensure safe and effective operation.

安全情報

• 顕微鏡は常に慎重に取り扱ってください。急激な動きや衝撃は避けてください。
• 顕微鏡が倒れないように、安定した水平な面に置いてください。
• 清掃やメンテナンスを行う前に、電源コードを抜いてください。
• 顕微鏡を直射日光、高温、高湿度にさらさないでください。
• 指定された電源アダプターのみを使用してください。
• Keep the optical components clean. Avoid touching lenses with bare hands.
• Do not attempt to disassemble or repair the microscope yourself. Contact qualified service personnel.

コンポーネントと機能

Familiarize yourself with the main components of your AmScope B120C-DK-E1 microscope:

図1: 以上view of the AmScope B120C-DK-E1 Binocular Compound Microscope, highlighting key features such as the 2-layer mechanical stage, 360-degree adjustable head, 40X-2500X magnification, coaxial coarse and fine focus, and LED sub-stage照明。

図2: The Siedentopf binocular head, featuring 360-degree rotation and a 30-degree inclination for comfortable viewing and posture adjustment.

図3: 詳細 view of the 2-layer mechanical stage and the coaxial coarse and fine focus knobs, designed for precise specimen movement and focusing.

図4: The LED sub-stage lighting system, providing cool, energy-efficient, and daylight-balanced illumination for improved contrast and resolution.

主要コンポーネント:

• Siedentopf Binocular Head: Rotates 360 degrees with a 30-degree inclination for ergonomic viewing。
• 接眼レンズ: Includes 10X and 25X widefield eyepieces for various magnification levels.
• 対物レンズ: Achromatic, color-coded objective lenses (e.g., 4X, 10X, 40X, 100X oil) mounted on a revolving nosepiece.
• メカニカルStage: A 2-layer stage with low-position controls for precise X-Y movement of slides.
• Coaxial Focus Knobs: Separate coarse and fine focus adjustments for accurate focusing.
• LED照明器具: サブtage LED light source with adjustable brightness.
• コンデンサー： Includes a brightfield condenser and a darkfield condenser for specialized observation.
• 1MP Digital Eyepiece Camera: For capturing images and videos, compatible with Windows, Mac, and Linux.

設定

1. 開梱: すべての部品をパッケージから慎重に取り出してください。パッケージは、将来の輸送や保管のために保管しておいてください。
2. 配置: 顕微鏡は直射日光や熱源から離れた、安定した振動のない場所に置いてください。
3. ヘッドの取り付け: Loosen the head retaining screw on the top of the microscope body. Gently insert the binocular head into the opening, ensuring it is fully seated. Tighten the retaining screw.
4. 接眼レンズの取り付け: Remove the protective caps from the eyepiece tubes. Insert the desired eyepieces (10X or 25X) into the tubes.
5. 対物レンズの取り付け: Rotate the revolving nosepiece to ensure there is no objective lens directly above the stage. Screw the objective lenses into the nosepiece in increasing order of magnification (e.g., 4X, 10X, 40X, 100X).
6. 電源接続: 電源コードを顕微鏡の電源入力に接続し、適切な電源コンセントに接続します。
7. Digital Eyepiece Camera Installation: If using the digital eyepiece, remove one of the standard eyepieces and insert the 1MP digital eyepiece camera into the eyepiece tube. Connect the camera to your computer via USB.

操作手順

1. Brightfield Observation

図5: Examples of magnification levels (10X, 100X, 400X, 1000X) achievable with the AmScope B120C-DK-E1, utilizing various objective lenses and eyepieces, including the additional 25X eyepieces for up to 2500X magnification.

1. 電源オン: Turn on the main power switch. Adjust the LED illuminator brightness using the dimmer control.
2. Stage 調整: 機械的なsを下げるtage using the coarse focus knob to create enough space to place a slide.
3. Slide Placement: 準備した標本スライドを機械のsに置くtage、sで固定するtage clips. Use the X-Y stage controls to position the specimen over the light aperture.
4. Magnification Selection: Rotate the revolving nosepiece to select the lowest power objective lens (e.g., 4X). Ensure it clicks into place.
5. フォーカス:
   • 接眼レンズを覗きながら、ゆっくりとsを上げますtag粗フォーカスノブを使用して、標本に大まかに焦点が合うまで操作します。
   • Use the fine focus knob to achieve sharp focus.
   • Adjust the interpupillary distance (distance between eyepieces) and diopter adjustment on the eyepiece for comfortable viewing and to correct for differences in vision between your eyes.
6. インクレasing Magnification: Once focused at low power, rotate the nosepiece to a higher power objective (e.g., 10X, 40X). The microscope is parfocal, meaning only minor adjustments with the fine focus knob should be needed.
7. Using 100X Oil Immersion Objective:
   • After focusing with the 40X objective, rotate the nosepiece halfway between the 40X and 100X objectives.
   • Place a small drop of immersion oil directly onto the center of the specimen slide.
   • Rotate the 100X objective into the oil drop. The objective lens should make contact with the oil.
   • Use only the fine focus knob to bring the image into sharp focus. Do not use the coarse focus knob with the 100X objective.
   • After use, clean the 100X objective lens and the slide immediately with lens cleaning solution and lens paper.
8. コンデンサー調整: Adjust the iris diaphragm on the condenser to control the amount of light and contrast. For higher magnifications, open the diaphragm more. For lower magnifications, close it slightly. The condenser can also be centered for optimal illumination.

2. Darkfield Observation

The AmScope B120C-DK-E1 includes a darkfield condenser for observing unstained, live specimens.

1. Condenser Exchange: Remove the brightfield condenser from its mount beneath the stage. Carefully insert the darkfield condenser into the mount. Ensure it is seated correctly.
2. 標本の準備: Darkfield observation works best with thin, unstained specimens.
3. 照明： Turn on the LED illuminator and set it to a high brightness level.
4. フォーカス: Use a low power objective (e.g., 10X or 20X, if available) first. Focus on the specimen as you would in brightfield. The specimen will appear brightly lit against a dark background.
5. Centering the Darkfield Condenser: Some darkfield condensers require centering. If your condenser has centering screws, adjust them until the darkfield effect is uniform across the field of view.
6. Higher Magnification: For higher magnifications, you may need to use a specialized darkfield objective or ensure your standard objectives are compatible. Always ensure sufficient immersion oil is used with 100X darkfield objectives.

3. Digital Eyepiece Camera Operation

The included 1MP digital eyepiece camera allows for image capture and live viewコンピューターで作業します。

図6: The AmScope B120C-DK-E1 microscope connected to a laptop, demonstrating live digital viewing and image capture capabilities via the 1MP digital eyepiece camera.

1. ソフトウェアのインストール: Install the professional microscopy software provided (or download the latest version from the AmScope website) onto your Windows, Mac, or Linux computer.
2. カメラを接続: Insert the digital eyepiece camera into one of the microscope's eyepiece tubes and connect it to your computer via the USB cable.
3. ソフトウェアの起動: Open the installed microscopy software. The software should detect the camera automatically.
4. ライブ View: The software will display a live feed from the microscope. Adjust microscope focus and illumination for the best image.
5. 画像/ビデオキャプチャ: Use the software's functions to capture still images or record videos of your observations. The software also provides tools for image processing and measurement.

メンテナンス

• レンズのクリーニング: Use a soft brush to remove dust from lens surfaces. For smudges or oil, use a small amount of lens cleaning solution on lens paper. Wipe gently in a circular motion. Never use abrasive cloths or harsh chemicals.
• クリーニングボディ: 顕微鏡本体を柔らかくて乾いた布で拭いてください。amp 布で拭いてください。電気部品に水分が入らないようにしてください。
• ストレージ： When not in use, cover the microscope with a dust cover to protect it from dust and debris. Store in a cool, dry place.
• 浸漬油: Always clean immersion oil from the 100X objective and slides immediately after use. Dried oil can be difficult to remove and can damage the lens.

トラブルシューティング

仕様

保証とサポート

The AmScope B120C-DK-E1 microscope comes with a 5年間のメーカー保証. For warranty claims, technical support, or service inquiries, please contact AmScope customer service directly.

For additional resources, software downloads, and frequently asked questions, please visit the official AmScope webサイト：

詳しくはこちら