1. はじめに
The AmScope D120A-MS is a dual-view compound monocular microscope designed for high magnification observation in biological and educational applications. It features a versatile viewing head, multiple objective lenses, Brightfield illumination, and a mechanical stage for precise specimen manipulation. This manual provides essential information for the proper setup, operation, and maintenance of your microscope.
2. 安全ガイドライン
- 顕微鏡は常に慎重に取り扱ってください。急激な動きや衝撃は避けてください。
- 顕微鏡が倒れないように、安定した水平な面に置いてください。
- 電源コードを正しい電圧のアース付きコンセントに接続します。tage（110V）。
- Do not touch optical surfaces with bare hands. Use lens paper or a soft, lint-free cloth for cleaning.
- Unplug the microscope from the power source before cleaning or performing any maintenance.
- 顕微鏡は直射日光、高温、ほこり、腐食性化学物質から遠ざけてください。
- Supervise children or inexperienced users to ensure safe operation.
3. パッケージ内容
すべてのコンポーネントを慎重に開梱し、次のアイテムが含まれていることを確認します。
- AmScope D120A-MS microscope with mechanical stage
- WF10x eyepiece
- WF16x eyepiece
- 4倍DINアクロマート対物レンズ
- 10倍DINアクロマート対物レンズ
- 40xS DIN achromatic objective (spring-loaded)
- 100xS (oil) DIN achromatic objective (spring-loaded)
- (2) Color filters
- 予備球
- ダストカバー
- 取扱説明書（本書）
4.コンポーネントの識別
Familiarize yourself with the main parts of your AmScope D120A-MS microscope:
図4.1: 以上view of the AmScope D120A-MS microscope. This image displays the complete microscope assembly, including the base, stage, objective lenses, and dual-view monocular head.
- 接眼レンズ（接眼レンズ）: どこを見るか view the specimen. The D120A-MS includes WF10x and WF16x eyepieces.
- 単眼鏡 Viewingヘッド： The upper part of the microscope containing the eyepiece tube. This model features a dual-view head with a fixed 45-degree inclined tube and a vertical port.
- 回転式ノーズピース: Holds the objective lenses and allows for easy switching between magnifications.
- 対物レンズ: The primary magnification components (4x, 10x, 40xS, 100xS oil).
- メカニカルStage: A platform where the specimen slide is placed, with controls for precise X-Y movement.
- Stage クリップ: Secure the specimen slide on the mechanical stage.
- 粗動フォーカスノブ: Large knob for rapid focusing adjustments.
- 微調整フォーカスノブ: Small knob for precise focusing adjustments.
- Illuminator (Light Source): sの下に位置tage, provides light for viewing. This model uses variable-intensity tungsten illumination.
- アッベコンデンサー: Located below the stage, focuses light onto the specimen. Features a 1.25 NA and an iris diaphragm.
- 虹彩絞り: Controls the amount of light passing through the specimen.
- ベース： The stable bottom support of the microscope.
5. 組み立てとセットアップ
Follow these steps to set up your microscope for initial use:
- 開梱: 顕微鏡とすべての付属品をパッケージから慎重に取り出してください。将来の輸送や保管のために、元のパッケージは保管しておいてください。
- 配置: Place the microscope on a sturdy, level surface, away from vibrations and direct sunlight.
- 接眼レンズの取り付け: Insert the desired eyepiece (WF10x or WF16x) into the inclined eyepiece tube of the monocular head. Gently twist to secure it.
- 目的のインストール: The objective lenses are typically pre-installed. If not, carefully screw each objective into the revolving nosepiece in increasing order of magnification (4x, 10x, 40xS, 100xS). Ensure they are finger-tight.
- 電源接続: Connect the power cord to the microscope's power input and then to a standard 110V electrical outlet.
- ダストカバー: 使用していないときは、顕微鏡を付属のダストカバーで覆い、ほこりやごみから保護してください。
図5.1: 閉じる view of the objective lenses mounted on the revolving nosepiece. This image shows the 4x, 10x, 40xS, and 100xS (oil) objectives in position.
6. 基本操作
This section outlines the fundamental steps for observing specimens with your microscope.
- 電源オン: Flip the power switch located on the base of the microscope. The illuminator should light up.
- 照明を調整する: Use the variable intensity control knob to set the desired brightness. Adjust the iris diaphragm on the Abbe condenser to optimize contrast and resolution for your specimen.
- 標本の場所: Rotate the revolving nosepiece to select the lowest power objective (4x). Lower the mechanical stage using the coarse focus knob. Place a prepared specimen slide onto the mechanical stageとsで固定しますtageクリップ。
- Position Specimen: XYを使用するtage control knobs to move the slide until the area of interest is centered under the objective lens.
- フォーカス:
- While looking through the eyepiece, slowly raise the stageを使用して 粗調整ノブ until the specimen comes into rough focus.
- 使用 微調整ノブ to achieve a sharp, clear image.
- 倍率の変更: Once focused at low power, rotate the revolving nosepiece to switch to a higher power objective (e.g., 10x, 40xS). Due to the parfocal design, only minor adjustments with the fine focus knob should be needed to bring the image back into sharp focus.
- Using the 100xS Oil Immersion Objective:
- After focusing with the 40xS objective, rotate the nosepiece halfway between the 40xS and 100xS objectives.
- Place a small drop of immersion oil directly onto the center of the specimen slide where the light passes through.
- Carefully rotate the 100xS objective into the oil drop until it clicks into place. The objective should be immersed in the oil.
- のみを使用してください 微調整ノブ to bring the image into sharp focus. Do not use the coarse focus knob with the 100xS objective.
- After use, clean the 100xS objective and the slide immediately with lens paper moistened with lens cleaning solution to remove all immersion oil.
- デュアル-View 機能性: The vertical port on the monocular head can accept a second eyepiece for simultaneous viewing by another person or a camera mount for digital image capture (eyepiece and camera sold separately). Dioptric adjustment on the upright tube accommodates individual eye-strength differences.
図6.1: The AmScope D120A-MS microscope demonstrating its digital image capture capability by connecting to a laptop. This setup utilizes the vertical port for a camera attachment.
7. メンテナンスとケア
適切なメンテナンスを行うことで、顕微鏡の寿命が長くなり、最適なパフォーマンスが確保されます。
- レンズのクリーニング: Use only specialized lens paper and lens cleaning solution for optical surfaces. Gently wipe in a circular motion. Never use abrasive cloths or harsh chemicals.
- 一般的な清掃: 顕微鏡本体を柔らかくて乾いた布で拭いてください。amp 布で拭いてください。電気部品に水分が入らないようにしてください。
- ストレージ： Always cover the microscope with the dust cover when not in use. Store it in a dry, dust-free environment.
- 電球の交換: If the illuminator bulb burns out, unplug the microscope. Refer to the base of the microscope for access to the bulb compartment. Carefully replace the old bulb with the provided spare bulb, ensuring not to touch the glass part of the new bulb with bare hands.
- 機械部品: Do not attempt to lubricate or disassemble any mechanical parts. If issues arise, contact customer support.
8。 トラブルシューティング
このセクションでは、発生する可能性のある一般的な問題について説明します。
| 問題 | 考えられる原因 | 解決 |
|---|---|---|
| 照明なし | Power cord disconnected, power switch off, bulb burnt out | Check power connection, turn on switch, replace bulb |
| 画像がぼやけている、または焦点が合っていない | Incorrect focus adjustment, objective not fully engaged, dirty lens | Adjust coarse/fine focus, rotate nosepiece until objective clicks, clean lenses |
| 暗視野 view | Iris diaphragm closed, illuminator too dim, condenser too low | Open iris diaphragm, increase illuminator brightness, adjust condenser height |
| ほこりや汚れ view | Dust on eyepiece, objective, or slide | Clean eyepiece, objective, or slide with lens paper |
| Cannot focus at 100x | No immersion oil, air bubbles in oil, coarse focus used | Apply immersion oil, remove air bubbles, use only fine focus |
9. 技術仕様
Detailed specifications for the AmScope D120A-MS microscope:
- 頭： Compound dual-view monocular, 45-degree inclined, 360-degree rotatable
- 接眼レンズ: WF10x, WF16x (with pointer)
- 目的: 4X, 10x, 40xS (spring), 100xS (oil, spring) DIN achromatic
- 倍率範囲: 40倍 - 1600倍
- Stage: 機械的tage with X-Y axis movement, stag停止
- 照明構成： 送信（下）
- コンデンサー： Abbe, 1.25 NA
- ダイヤフラム: 虹彩絞り
- 光源： Tungsten (incandescent), variable intensity
- 照明タイプ: ブライトフィールド
- 力： 110V
- 材料： Metal, tungsten
- 寸法（高さ×幅×奥行き）： 15 x 7.5 x 5.125インチ（約）
- 重さ： 10ポンド
10. 保証と顧客サポート
For warranty information, technical assistance, or to purchase replacement parts, please contact AmScope customer support. Refer to the AmScope web現在の連絡先の詳細については、サイトまたは購入書類をご覧ください。